水稻产量对长期不同施肥和环境的响应

以开始于1982年的不同施肥长期定位试验为平台,选择氮磷钾肥(NPK)、有机肥(牛粪,M)、氮磷钾肥+有机肥(NPKM)、氮磷肥+有机肥(NPM)、氮钾肥+有机肥(NKM)和磷钾肥+有机肥(PKM)共6个处理,采用产量年际变化、变异系数(CV)、产量可持续指数(SYI)、肥料增产贡献率及AMMI模型对影响双季稻稻谷总产量稳定性的施肥处理、环境和二者互作进行综合分析,研究历年稻谷产量(1982—2012年)对长期不同施肥和环境的响应特征。结果表明:NPKM处理稻谷产量最高;在养分投入量相同的情况下,单施有机肥和单施化肥对于早稻产量的影响效果一致,但单施有机肥较单施化肥有利于提高晚稻稻谷产量。不同施肥处理稻谷产量CV随试验时间延长逐渐降低,有机无机配施相比较单施化肥能够减小产量CV,各处理晚稻产量的CV大于早稻,但其SYI值小于早稻。NPKM处理的SYI值最高,为0.51;M和NPK处理的SYI值分别为0.44和0.42。肥料对于产量的贡献率表现为:有机肥化肥氮化肥磷化肥钾。AMMI模型交互效应主成分(IPCA)表明,不同施肥处理在不同试验年份对环境的响应不一样。综合以上分析结果,氮磷钾完全肥基础上配施有机肥(NPKM)是该区域双季稻高产和稳产的最佳施肥措施。     

腐殖酸结合汞对多年生植物(Citrus Sinensis L. Osbeck)的生物有效性初探

野外采集广柑树及供其生长的土壤,研究多年生植物对土壤Hg的吸收及与土壤理化性质、腐殖酸结合汞（HS—Hg）的关系。结果表明,在酸性土壤环境中,酸性过强,果树吸收Hg量会更低;果实部分Hg含量与土壤的有机质或腐殖酸含量呈现负相关关系;HS—Hg特别是FA—Hg（富啡酸结合汞）组分是果树吸收、积累Hg的重要来源,其与根Hg的相关系数达到0．700^＊-0．759^＊＊,且以表层土壤更能提供有效的HS—Hg。     

Effects of the urease inhibitor N-(n-butyl)phosphorothioic triamide in low concentrations on ammonia volatilization and evolution of mineral nitrogen

Laboratory incubation experiments were conducted to study the influence of increasing concentrations of N-(n-butyl)phosphorothioic triamide (NBPT) on NH₃ volatilization and rate of urea hydrolysis and evolution of mineral N in Ozzano, Rimini and Carpi soils with different physicochemical characteristics. Low concentrations of NBPT reduced NH₃ losses due to volatilization after urea fertilization and the effectiveness of the inhibitor was related to the soil characteristics (e.g. high concentrations of organic C and sand). After 15 days of incubation, no significant reductions of losses were found for any of the NBPT concentrations employed in Rimini soil. The application of NBPT led to a considerable reduction of the formation of nitrite. This process was completely annulled with the highest dose of NBPT (0.5% w/w_urea) in the Carpi soil after 15 days. In Rimini soil, however, the use of NBPT was less effective in influencing nitrite formation. The use of NBPT favoured accumulation of nitrate proportional to the NBPT concentration employed while it had no influence on the NH _inf4 ^sup+ fixation by 2:1 layer silicates. The data obtained support previous evidence that NBPT is effective in reducing the problems encountered in using urea as fertilizer. However, environmental conditions and soil physicochemical characteristics may have an important influence on the effectiveness of NBPT.     

Molybdenum reserves of seed,and growth and N2 fixation by Phaseolus vulgaris L.

Summary Plants grown from seed with high (1.5–7.3 g Mo seed^-1) and low (0.07–1.4 g Mo seed^-1) Mo contents were grown in the presence and absence of Mo in growth media (perlite) or in a flowing-solution culture, in a controlled environment. Neither the high (1.5 g Mo seed^-1) nor the low (0.1 g Mo seed^-1) Mo content in seed from a small-seeded genotype (BAT 1297) was able to prevent Mo deficiency (reduced shoot, root and nodule dry weight, N₂ fixation and seed production) in growth media without an external supply of Mo, whereas both the high (7.3 g Mo seed^-1) and the low (0.07 g Mo seed^-1) contents in seed were able to prevent Mo deficiency in a large-seeded genotype (Canadian Wonder). Responses to Mo treatment by the Two genotypes were inconsistent between the growth media and solution culture experiments. Seed with a large Mo content (3.5 g Mo seed^-1) from the Canadian Wonder genotype was unable to prevent Mo deficiency (reduced shoot and nodule dry weight and N₂-fixation) in a solution culture without an external source of Mo, whereas both the large (1.7 g Mo seed^-1) and the small (0.13 g Mo seed^-1) contents in seed prevented a deficiency in BAT 1297. Growing plants from seed with a small Mo content, without additional Mo, reduced the seed Mo content by 83–85% and seed production by up to 38% in both genotypes. Changes in seed size and increases in shoot, root and nodule dry weight occurred, but varied with the genotype and growth conditions. These effects were also observed in some cases where plants were grown with additional Mo, demonstrating that the amount of Mo in the seed sown can influence plant nutrition irrespective of the external Mo supply. Nodule dry weight, total N content of shoots and seed production were improved by using seed with a small Mo content (1.64–3.57 g Mo seed^-1) on acid tropical soils in Northern Zambia. Plants of both the large- and small-seeded genotypes grown from seed with a small Mo content (<1.41 g Mo seed^-1) had a smaller nodule weight, accumulated less N and produced less seed. The viability of seed with a small Mo content was lower (germination up to 50% less) than that of seed with a large Mo content.     

广西早籼品种(组合)的稻米品质状况及改良目标

广西多数早籼品种(组合)的糙米率、精米率、粒长、粒形、糊化温度、胶稠度、蛋白质含量等指标。达到了农业部(NY122-86)二级优质食用稻米标准。但完整米率的达标率偏低,胚乳的垩白程度偏大,垩白粒率偏高,直链淀粉含量偏高,米粒的蒸煮延长性差。今后,早籼稻的品质育种应把提高完整米率,降低直链淀粉含量和提高胚乳的透明度作为品质改良的主攻目标。     

Effect of high temperature on growth,nitrogen fixation,and chlorophyll content of five species of Azolla Anabaena symbiosis

Summary Cultures of Azolla sp. ST-SI, A. microphylla BR-GI, A. mexicana BR-GL, A. caroliniana WT-V, and A. filiculoides BR -H were grown in N-free International Rice Research Institute growth medium in the glasshouse at 38±1 °C (day) and 25±1 °C (night) under a light intensity of 350 Em²s^–1 for 27 days. Biomass, chlorophyll contents and nitrogenase activity (acetylene reduction assay) were recorded on the 19th and 27th day. For comparison the same parameters were studied in Azolla spp. under normal growth conditions at 26±1 °C (day) and 19±1 °C (night). Azolla sp. STSI and A. microphylla BR-GI had produced a larger biomass by the 19th and the 27th day of incubation than A. caroliniana WTV and A. filiculoides which showed poor growth. Under normal growth conditions A. caroliniana WTV and A. filiculoides BRH produced less biomass than the other Azolla spp. cultures tested. A. mexicana BR-GL had a higher total chlorophyll content in both incubation periods than A. caroliniana WT-V and A. filiculoides BR-H. The N content was high in Azolla sp. ST-SI, A. microphylla BR-GI, and A. mexicana BR-GL compared with the low N content of A. filiculoides BR-H and A. caroliniana WT-V. At the higher temperature (38±1 °C/25±1 °C) Azolla sp. ST-SI and A. microphylla BR-GI consistently showed a higher growth rate than A. filiculoides BR-H and A. caroliniana WTV, while the growth rate of A. mexicana BR-GL was intermediate.The study was carried out at C.F. Kettering Research Laboratory, Yellowsprings, OH - 45387, USA     

The effect of phenyl phosphorodiamidate on urease activity and ammonia volatilization in flooded rice

Summary The behaviour of urease activity, ammoniacal N concentrations and pH in flood water and that of ammonia flux was investigated in a water-logged soil either in the presence or in the absence of rice and with three different treatments (control, urea and urea + phenyl phosphorodiamidate). In the presence of the phenyl phosphorodiamidate (PPD), that is a urease inhibitor, increases in ammoniacal N concentrations and in ammonia evolution were delayed but not eliminated. The degradation and/or the inactivation of PPD might have occurred, thus removing the inhibition of the enzyme activity.     

Free and HF-HO-extractable amino acids determined by high performance liquid chromatography in a loamy sand soil

Summary Amino acids were extracted from fertility plots of a loamy sand soil with 0.05 M HF-HCl and with a 10% ethanol solution (free amino acids) and analysed by reverse-phase high performance liquid chromatography (HPLC). The total quantities of amino-N compounds analysed were 4.4 g/g soil for the acid treatment and 22.6 g/g soil for the 10% ethanol extract. Glycine and glutamic acid were the most abundant of 15 amino-N compounds in the HF-HCl extracts, whereas glutamic acid and ornithine + NH⁺ ₄ were found in the highest concentration in the 10% ethanol extracts. The HF-HCI pretreatment is used to increase the efficiency of the extraction of soil organic matter. Although this pretreatment removed some amino acids, the acids extract less than 1% of the total amino-N content of the crude soil extracts. The pretreatment, therefore, was not overly destructive. Comparisons between the amino acids extracted from the fertility plots were not conclusive, except for glycine, which was greater in concentration in the higher fertilizer N plots of the same crop rotation.     

Trade-offs between on-farm varietal diversity and highly client-oriented breeding — a case study of upland rice in India

On-farm varietal diversity is now highest in marginal agricultural environments because, unlike the case in more favourable areas, modern varieties have not been a sufficiently attractive option for farmers to replace their landraces. However, the continued survival of landraces on farm is dependent on the continuing failure of plant breeding to provide better alternatives. Highly client-oriented breeding can produce suitable modern varieties for areas that were dominated by landraces. We examine here the case of the adoption of two upland rice varieties in three states in eastern India, Jharkhand, West Bengal and Orissa, where our surveys had confirmed low adoption of modern varieties in the upland ecosystem. The two new upland varieties were readily accepted by farmers who rapidly adopted them on large proportions of their land. On-farm diversity is maintained by the need to trade-off among varieties but once a variety with overall superiority was found this incentive was removed. The new varieties from the client-oriented breeding did not have weaknesses that farmers had to trade-off against their landraces so they were more likely to replace them. They also replaced older modern varieties. Farmers had previously maintained them along with landraces because they had to trade-off the higher yield of Kalinga III against the more stable yield of landraces or the higher yield of Vandana against the higher gain quality of the landraces. Only mean count per household could be used to test the significance of the differences between individual years. In all states, this revealed a significant reduction in landrace diversity with the adopting farmers when the addition of the two new varieties was not considered. Trends across years for mean varietal count, total count and Shannon–Wiener index showed a significant decline in Orissa, irrespective of whether the two new varieties were included in the analysis or not. In Jharkhand there was a significant decline only for mean count when the new varieties were not included. In Orissa, it was possible to test if rare landraces were as readily replaced as more common ones. Landrace replacement was unmitigated by increasing rarity yet the most rare landraces have the highest priority for genetic conservation. Some landraces were completely replaced by the farmers in the sample despite a diverse variety portfolio being a risk-reducing strategy in a region where there is a high risk of crop failure. There was little environmental heterogeneity in the marginal, drought-prone areas to slow this decline. The new varieties had an impact on the farming system because sometimes farmers brought additional upland into cultivation. They also introduced these varieties into more favourable, medium land. The strategy of releasing two new upland varieties met with some success in maintaining diversity.